Higher order structure in a short repeat length chromatin.
نویسندگان
چکیده
منابع مشابه
Higher order structure in a short repeat length chromatin
Polynucleosomes from calf brain cortical neurone nuclei have an average repeat length of less than 168 base pairs. The ability of this material to adopt higher order structure has been assessed by various physical techniques. Although containing on average less DNA per nucleosome than is required to form a chromatosome, this short repeat length chromatin folded in an H1 dependent manner to a st...
متن کاملMethods for identifying higher-order chromatin structure.
Eukaryotic genomic DNA is combined with histones, nonhistone proteins, and RNA to form chromatin, which is extensively packaged hierarchically to fit inside a cell's nucleus. The nucleosome-comprising a histone octamer with 147 base pairs of DNA wrapped around it-is the initial level and the repeating unit of chromatin packaging, which electron microscopy first made visible to the human eye as ...
متن کاملChromatin higher-order structure and dynamics.
The primary role of the nucleus as an information storage, retrieval, and replication site requires the physical organization and compaction of meters of DNA. Although it has been clear for many years that nucleosomes constitute the first level of chromatin compaction, this contributes a relatively small fraction of the condensation needed to fit the typical genome into an interphase nucleus or...
متن کاملChromatin Higher Order Structure and Chromosomal Positioning in the Nucleus
While it has been known for some time that chromatin is highly packaged, it has only recently been discovered that chromatin is attached to the nuclear matrix. It has also been recently shown that interphase chromosomes remain separate from one another in so called, “chromosome domains.” This author suggests a functional relationship between chromatin attachment to the nuclear matrix and chromo...
متن کاملBiochemical evidence of variability in the DNA repeat length in the chromatin of higher eukaryotes.
Biochemical evidence is presented which confirms that the DNA repeat length in micrococcal nuclease (spleen endonuclease, nucleate 3'-oligonucleotidohydrolase, EC 3-1-4-7) digests of Chinese hamster ovary chromatin is shorter than that of rat liver chromatin [J.L. Compton, R. Hancock, P. Oudet, and P. Chambon (1976) Eur. J. Biochem., in press]. A survey of available cells has shown that the DNA...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1984
ISSN: 0021-9525,1540-8140
DOI: 10.1083/jcb.98.4.1320